一、介绍Introduction:
膜再生液用于破坏膜结合蛋白和一抗之间的相互作用,使膜可重复使用。使用这种剥离缓冲液,膜一般可重复使用3-5次。然而,同一膜的多次重复使用会导致蛋白质信号减弱。
Western Stripping Buffer is used to disrupt the interaction between membrane-bound proteins and primary antibodies to make the membrane reusable. Membranes can be reused 3-5 times generally with this stripping buffer. However, multiple reuse of the same membrane can lead to weakened protein signals.
二、实验流程:Procedure:
1.Western化学发光试验完成后,用TBST冲洗膜5分钟。
After completion of the Western chemiluminescence assay, rinse membranes in TBST for 5 minutes.
2.丢弃TBST,加入足够的膜再生液以完全覆盖膜。摇晃冲洗10-20分钟。
Discard the TBST and add sufficient Western Stripping Buffer to cover membranes completely. Rinse for 10-20 minutes with shaking.
3.弃去膜再生液,用TBST洗涤膜3次,振荡3-5分钟。
Discard the Western Stripping Buffer and wash membranes 3 times with TBST for 3-5 minutes with shaking.
4.进行膜封闭和其他后续程序。
Proceed to membrane blocking and other subsequent procedures.
三、数量Quantity:
125mL/250mL
四、研究用途Research Use:
仅用于研究用途,不适用于诊断程序。
For research use only, not for use in diagnostic procedures.
五、存储和稳定性Storage & Stability:
2-8℃下可储存12个月。
Store at room temperature for up to 12 Months.