Caspase 3 (D146) polyclonal antibody

BackGround:

Caspase 3 (also known as CPP32, YAMA and apopain) is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as in- active pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other up- stream proteases (e.g. Caspases 8, 9 and 10).The pro- cessed form of Caspase 3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 - Ser29 and Asp175 - Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF).

Product:

Rabbit IgG in PBS with 0.02% sodium azide,50% glycerol, pH7.2

Molecular Weight:

~ 35 kDa

Swiss-Prot:

P42574

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope specific immunogen and the purity is > 95% (by SDS-PAGE).

Applications:

WB: 1:250~1:500    IHC: 1:25~1:100   IF: 1:25~1:100

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C longterm. Avoid freeze-thaw cycles.