β-Actin (4D3) monoclonal antibody

BackGround:

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells.

Product:

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol,pH7.2

Molecular Weight:

~ 42 kDa

Purification&Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Applications:

WB: 1:5000~1:20000

IHC: 1:100~1:1000

IF: 1:100~1:500

IP: 1:100~500

Storage&Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:

β-Actin (4D3) detects endogenous levels of β-actin protein.

DATA:

IF image of BS6007M stained A549 cells.The cells were 4% para- formaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the anti- body β-Actin (4D3) #BS6007M(1:200) at 5µg/ml overnight at +4°C. The secondary antibody (Red) was Goat Anti-Mouse IgG (H+L) Rho- damine (TRITC)#BS11502 used at a 1/1000 dilution for 1h.Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).Immunoprecipitation of MCF-7 cell lysates using β-Actin mAb (Se- pharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG  Control (Sepharose Bead Conjugate)#BD0048 (lane 4and lane 5) .Lane 1 is 30% input. The western blot was probed using β-Actin mAb.

Note:

For research use only, not for use in diagnostic procedure.