β-Actin (I102) polyclonal antibody

产品货号：QS04025

规格规格：25µg，50µg，100µg

储存条件：4-8°C

产品优势：质优价廉

应用场景：WB，IP，IF

产品手册：

BackGround:

All eukaryotic cells express actin, which often constitutes asmuch as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. actin expression is limited to various types of muscle, whereas and actin are the principle constituents of filaments in other tissues.Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of ctin stress fibers and focal adhesion, Rac regulates actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

Product:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide,50% glycerol, pH7.2

Molecular Weight:

~ 42 kDa

Purification&Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Applications:

WB: 1:5000~1:20000

IP: 1:100~500

IF: 1:500~1000

Storage&Stability:

Store at 4°C short term. Ali quot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:

β -actin (I102) polyclonal antibody detects endogenous levels of β-actin protein.

DATA:

Western blot (WB) analysis of β-Actin(I102) pAb at 1:5000/1:10000/1:20000 dilution

Lane1:CT-26 whole cell lysate(40ug)

Lane2:PC12 whole cell lysate(40ug)

Lane3:HEK293T whole cell lysate(40ug)

Lane4:A549 whole cell lysate(40ug)

Immunofluorescence analysis of RAW264.7 cells using β-actin (I102) pAb at dilution of 1:500 (40x lens).

Immunoprecipitation of MCF-7 cell lysates using β-actin (I102) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspe- cific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4and lane 5) .Lane 1 is 30% input. The western blot was probed using β-Actin pAb.

Note:

For research use only, not for use in diagnostic procedure.

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β-Actin (I102) polyclonal antibody

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